Summary: Researchers have discovered a protein, neuritin, that is associated with cognitive resilience to Alzheimer’s disease and may delay cognitive decline.
This protein helps retain neuronal connections, even when toxic substances such as amyloid beta plaques and tau tangles that cause Alzheimer’s try to break them down.
- A protein called neuritin may help some people resist Alzheimer’s disease and cognitive decline.
- Neuritin is associated with intact cognitive function over time, and it may prevent the toxic substances that cause Alzheimer’s from breaking down neuronal connections and communication in the brain.
- Researchers plan to focus on the basic biology of neuritin and its potential as a biomarker or therapeutic for Alzheimer’s disease.
A recent study revealed that a protein important for neuron communication is associated with patient resistance to Alzheimer’s disease and may delay cognitive decline.
Like the cord that connects a telephone handset and the receiver, a neuron needs to make a physical connection with other neurons to communicate and transmit signals, which allow people to think and speak.
The new study published in the journal Molecular & Cellular Proteomics suggests a protein, called neuritin, may allow some people to retain their neuronal connections even when toxic substances that cause Alzheimer’s attempt to break them down.
Alzheimer’s is the most common cause of dementia and affects more than 5.8 million Americans. To diagnose it, physicians use mental competency tests, physical and neurological exams, brain imaging, spinal fluid tests and medical history.
Most patients show both a cognitive decline as well as toxic protein accumulation in the brain, which causes neuron death and brain shrinkage.
These abnormal protein aggregates, called amyloid beta plaques and tau tangles, can disrupt neuronal connections and communication, which leads to memory loss and confusion, the hallmark symptoms of Alzheimer’s.
However, some patients show characteristic signs of Alzheimer’s pathology in their brains when examined but remain mentally competent. These individuals are known as “cognitively resilient” by the researchers who conducted the study.
“How cognitively normal older individuals with Alzheimer’s disease pathology withstand dementia onset is one of the most pivotal, unanswered questions in the field,” said Jeremy Herskowitz, associate professor of neurology at the University of Alabama at Birmingham School of Medicine and co-supervisor of the project.
To tackle this question, Herskowitz and Nicholas Seyfried, a professor of biochemistry at Emory University School of Medicine and co-supervisor of the project, teamed up and combined their specialties in proteomics and basic neurology to examine proteins in human brain tissues.
Unlike widely employed hypothesis-driven research, this team studies diseased humans and their tissues first to discover potential therapeutics.
“That’s quite different than traditional approaches, which try to make discoveries in experimental model systems,” Herskowitz said.
“Our research collectively identifies differences in humans first. Then, after that discovery is made, we can ask questions in experimental model systems to work out what’s going on at the molecular and cellular level.”
The researchers conducted a large mass spectrometry screen of the proteins found in the brains of healthy people, typical Alzheimer’s patients and cognitively resilient patients. Cheyenne Hurst, a graduate student at Emory and co-lead author on the study, used high-powered computer programming to determine that neuritin correlates with intact cognitive function over time.
“The higher the amount of neuritin you have in your brain, the more likely you are to be cognitively intact,” Hurst said.
The researchers then wanted to test how the protein affects how neurons communicate. To do this, they isolated neurons from the hippocampus of rats and treated them with either neuritin, the pathogenic amyloid beta or both.
Derian Pugh, a graduate student at UAB and co-lead author, noticed structural differences in the three groups.
“The dendritic spines or synapses coming off healthy neurons kind of remind me of branches on a tree,” Pugh said.
But the structure of the neurons exposed to pathogenic amyloid beta was disrupted — and so were their connections with other neurons. Pugh said they “looked like a tree with no branches.”
However, neuritin completely blocked the detrimental effects of amyloid beta on the neuron cultures.
“With these experiments, we were able to recapitulate what happens in humans that display cognitive resilience and a possible mechanism,” Herskowitz said.
The team plans to focus on the basic biology of neuritin but also on how they can harness neuritin as a biomarker of Alzheimer’s or a therapeutic.
“The ability to estimate the amount of amyloid beta pathology in an older person’s brain using biomarkers is getting very advanced,” Seyfried said.
“We can predict quite accurately the presence of amyloid beta is in someone’s brain while they’re still alive. If they have a large amount of amyloid beta, but they’re still cognitively normal, they may want to one day get treated with neuritin or drugs that boost neuritin levels so that those symptoms don’t develop into dementia.”
About this Alzheimer’s disease research news
Original Research: Open access.
“Integrated Proteomics to Understand the Role of Neuritin (NRN1) as a Mediator of Cognitive Resilience to Alzheimer’s Disease” by Jeremy Herskowitz et al. Molecular & Cellular Proteomics
Integrated Proteomics to Understand the Role of Neuritin (NRN1) as a Mediator of Cognitive Resilience to Alzheimer’s Disease
- Multiplex tandem mass tag mass spectrometry on human frontal and temporal cortex.
- Computational network analysis links NRN1 with cognitive resilience to Alzheimer’s disease.
- NRN1 facilitated dendritic spine resilience against amyloid-β in cultured neurons.
- NRN1-induced proteome changes in cultured neurons associated with cognitive resilience.
The molecular mechanisms and pathways enabling certain individuals to remain cognitively normal despite high levels of Alzheimer’s disease (AD) pathology remain incompletely understood.
These cognitively normal people with AD pathology are described as preclinical or asymptomatic AD (AsymAD) and appear to exhibit cognitive resilience to the clinical manifestations of AD dementia.
Here we present a comprehensive network-based approach from cases clinically and pathologically defined as asymptomatic AD to map resilience-associated pathways and extend mechanistic validation.
Multiplex tandem mass tag mass spectrometry (TMT-MS) proteomic data (n=7,787 proteins) was generated on brain tissue from Brodmann area 6 and Brodmann area 37 (n=109 cases, n=218 total samples) and evaluated by consensus weighted gene correlation network analysis. Notably, neuritin (NRN1), a neurotrophic factor previously linked to cognitive resilience, was identified as a hub protein in a module associated with synaptic biology.
To validate the function of NRN1 with regard to neurobiology to AD, we conducted microscopy and physiology experiments in a cellular model of AD. NRN1 provided dendritic spine resilience against amyloid-β (Aβ) and blocked Aβ-induced neuronal hyperexcitability in cultured neurons.
To better understand the molecular mechanisms of resilience to Aβ provided by NRN1, we assessed how exogenous NRN1 alters the proteome by TMT-MS (n=8,238 proteins) of cultured neurons and integrated the results with the AD brain network. This revealed over-lapping synapse-related biology that linked NRN1-induced changes in cultured neurons with human pathways associated with cognitive resilience.
Collectively, this highlights the utility of integrating the proteome from human brain and model systems to advance our understanding of resilience-promoting mechanisms and prioritize therapeutic targets that mediate resilience to AD.